A good IVF Clinic routinely does Blastocyst Transfers for
all their patients. In order to ensure we have enough blastocysts, it’s very
important to ensure Good Quality Control .
We need to religiously maintain our equipment in proper
working conditions, which is why we check the CO2 Concentration, pH and
Temperature of our CO2 incubators regularly,
We maintain Log books, because an incubator which functions
properly is critical as far as fertilization , Embryo growth and Blasotcyst
conversion is concerned. This is why it
is very important to check that that all equipments work properly, as even the
slightest of variation can affect the Blastocyst conversion rate.
Good IVF Clinics have Skilled Embryologists, who use a CO2
analyzer, ph meter and a digital Thermometer in their Lab, which helps them to keep
their Incubators working properly.
Co2 Analyzer
Picture of CO2
incubator
Frequency of Co2
Checking
Co2 Concentration can be checked once in a week , and mandatorily
after cleaning, decontaminating and restarting the Incubator.
We also make sure that we change the Co2 Cylinders once they
are half empty, as there are very high chances of the Co2 cylinder having Impurities
at the bottom half of the Cylinder, which can have an effect on the embryo
growth and Embryo Quality.
Thermometer
Digital
Thermometer
We use a digital Thermometer to Check the temperature of all
our equipments, including the incubator and the surface of all our heated
stages. Embryos need to be pampered to keep them happy !
Digital pH Meter
We use a digital ph meter to check the pH of the Culture
media. It needs 3 point Calibration
before use.We use 3 buffers to calibrate it , and make sure that it works well
before we check the pH of culture media. The 26.0C refers to room temperature
on this screen.
Frequency of ph
Checking
We should Check the pH Mandatorily for every new batch of
Culture media, and after cleaning and restarting the Co2 incubator .
IVF labs without their own CO2 Analyzer, pH meter and
digital Thermometers, would never be confident about their Blastocyst
Conversion rate. This is because incubators are electronic equipment , and
their CO2 concentration and temperature are bound to vary over time.
This is why it is important to check the CO2 , pH and
Temperature of all equipment to get optimum fertilization and blastocyst
conversion rate.
The best bio-assay is to monitor the blastocyst conversion
rate, and at Malpani Infertility Clinic we expect this to be around 30 -50% .
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